Pouch Method for Isolating and Enumerating Propionibacteria

Membrane filter method for enumerating Escherichia coli.

A membrane filter procedure for enumerating Escherichia coli was developed and evaluated. The method quantifies E. coli within 24 h without requiring subculture and identification of isolates. It incorporates a primary selective-differential medium for gram-negative, lactose-fermenting bacteria; resuscitation of weakened organisms by incubation for 2 h at 35 degrees C before incubation at 44.5 ...

New medium for isolating propionibacteria and its application to assay of normal flora of human facial skin.

The conditions for isolation and cultivation of Propionibacterium acnes and related propionibacteria were studied in detail. Triton X-100 added to the diluent inhibited the growth of propionibacteria in concentrations of 0.05 to 0.1%. However, such was not the case with Tween 80; rather, growth of the bacteria was further enhanced by this agent. Consequently, Tween 80 was considered to be a sui...

A simple method for isolating individual microbes.

A New Method for Isolating I-(+)-lysine

None of the methods which have been used for isolating Z-(+)lysine from plant extracts and protein hydrolysates is ideal. The oldest method utilizes phosphotungstic acid to precipitate the basic nitrogenous compounds which must be fractionated subsequently (1, 2). In the Kossel-Kutscher silver sulfate-barium hydroxide fractionation (3) the lysine remaining in the final filtrate must be precipit...

A rapid method for isolating glandular trichomes.

A physical method is described for the rapid isolation of plant trichomes, with emphasis on stalked glandular types. The technique involved breaking frozen trichomes with powdered dry ice and collection of glandular heads by sieving from larger tissue fragments. This method was applied to several plants that bear similar stalked trichomes: geranium (Pelargonium), potato (Solanum tuberosum), tom...